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ief buffer concentrate  (Cytiva Europe)


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    Structured Review

    Cytiva Europe ief buffer concentrate
    Ief Buffer Concentrate, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 3315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ief+system/IPG+buffer/pm41742785-135-59-64
    Average 95 stars, based on 3315 article reviews
    ief buffer concentrate - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Electrofocusing:

    Article Title: Proteomic analysis of flavokawain C-induced cell death in HCT 116 colon carcinoma cell line.
    Article Snippet: Accepted Manuscript Proteomic analysis of flavokawain C-induced cell death in HCT 116 colon carcinoma cell line Chung-Weng Phang, Nur Ashikhin Gandah, Sri Nurestri Abd Malek, Saiful Anuar Karsani PII: S0014-2999(19)30266-3 DOI: https://doi.org/10.1016/j.ejphar.2019.04.032 Reference: EJP 72351 To appear in: European Journal of Pharmacology Received Date: 26 December 2018 Revised Date: 12 April 2019 Accepted Date: 15 April 2019 Please cite this article as: Phang, C.-W., Gandah, N.A., Abd Malek, S.N., Karsani, S.A., Proteomic analysis of flavokawain C-induced cell death in HCT 116 colon carcinoma cell line, European Journal of Pharmacology (2019), doi: https://doi.org/10.1016/j.ejphar.2019.04.032.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Apparent degradation forms of rhG-CSF under forced conditions: Insights for better quality-control of bioproducts.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Proteomic Analysis of G2/M Arrest Triggered by Natural Borneol/Curcumin in HepG2 Cells, the Importance of the Reactive Oxygen Species-p53 Pathway.
    Article Snippet: Curcumin (Cur), an active ingredient from the rhizome of the plant, 24 Curcuma longa, has wide anticancer activities.. However, due to its poor solubility and 25 hence poor absorption, Cur has limited clinical applications.. It is therefore important 26 to develop an effective method to improve its absorption.

    Article Title: Immunoproteomic Profiling of Bordetella pertussis Outer Membrane Vesicle Vaccine Reveals Broad and Balanced Humoral Immunogenicity.
    Article Snippet: Immobiline 156 DryStrip pH 3-10NL, 7 cm (GE Healthcare) were rehydrated (ON, RT) with protein sample 157 in an IPGbox (GE Healthcare). .. Isoelectric focusing (IEF) was performed in an Ettan IPGphor 158 3 IEF system (GE Healthcare) according to the following conditions: 0.5 h at 300 V, 0.5 h at 159 1000 V, 1 h at 2000 V, 1 h at 3000 V, 1 h at 4000 V, and 1 h at 5000 V. After IEF, strips were 160 equilibrated in 3 ml equilibration buffer (75 mM Tris-HCl pH 8.8, 6 M urea, 30% glycerol, 161 2% SDS, and bromophenol blue) with DTT (65 mM) for 15 min. at RT, followed by 3 ml 162 equilibration buffer with iodoacetamide (54 mM) for 15 min. at RT. .. 163 164 Gel electrophoresis 165 For SDS-PAGE, 10 μg B. pertussis (B1917) lysate, 1 μg of Prn P.69 (in house prepared (36)), 166 Ptx (Kaketsuken, Japan), FHA (Kaketsuken, Japan), or Fim2/3 (Sanofi) were incubated (10 167 min., 100°C) with 3.3 μl reducing sample buffer (250 mM Tris, 8% SDS, 400 mM DTT, 40% 168 Glycerol, 0.04% bromophenol blue) and loaded on a 10% NuPAGE bis tris 1.0-mm precast 169 gel (Invitrogen).

    Article Title: Detection of orthodontically induced inflammatory root resorption-associated biomarkers from the gingival crevicular fluid by proteomics analysis: a randomized-controlled clinical trial.
    Article Snippet: .. Ettan IPGphor II Isoelectric Focusing (IEF) System and 7 cm immobile pH gradient strips (IPG) (GE Healthcare, Chicago, IL, USA), pH 4–7, were used. ..

    Article Title: Proteomic and Systematic Functional Profiling Unveils Citral Targeting Antibiotic Resistance, Antioxidant Defense, and Biofilm-Associated Two-Component Systems of Acinetobacter baumannii To Encumber Biofilm and Virulence Traits
    Article Snippet: Prior to isoelectric focusing (IEF), six immobilized pH gradient (IPG) strips (Immobiline DryStrip pH 4 to 7, 18 cm) were rehydrated for 12 h at 20°C by adding 350 μl (for each strip) of rehydration buffer (7 M urea, 2 M thiourea, 2% CHAPS, 0.5% IPG buffer [pH 4 to 7], 0.002% bromophenol blue, and 12.5 mg/ml Destreak reagent [GE Healthcare]) containing 500 μg of protein extracted from the control/citral-treated sample per strip (biological triplicates). .. After rehydration, IPG strips were placed on mineral oil in an IEF system (Ettan IPGphor 3; GE Healthcare), and IEF was carried out by applying a 75-μA current per IPG strip under the following conditions: 100 V (2-h step), 100 to 500 V (3-h gradient), 500 V (2-h step), 500 to 5,000 V (3-h gradient), 5,000 V (2-h step), 5,000 to 8,000 V (3-h gradient), 8,000 V (2-h step), 8,000 to 10,000 V (3-h gradient), and 10,000 V (2-h step) at 20°C for 22 h. Prior to two-dimensional gel electrophoresis (2DGE), the IPG strips were equilibrated with two different equilibration buffers, equilibration buffer I (6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 1% DTT in 50 mM Tris-HCl buffer [pH 8.8]) for 15 min and equilibration buffer II (6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 2.5% iodoacetamide [IAA] in 50 mM Tris-HCl buffer [pH 8.8]) for 15 min. Next, equilibrated IPG strips were placed on top of 12% gels and sealed with a 0.3% agarose solution. .. The Ettan DALTsix electrophoresis system (GE Healthcare) was used for electrophoresis under the following program conditions: 100 V (200 mA) for 1 h and 150 V (300 mA) for 8 h. After electrophoresis, the gels were fixed, stained by CBB according to the above-described method, and imaged at a 300-dpi (dots per inch) resolution using an Image Scanner III system (GE Healthcare) equipped with LabScan 6.0 software ( ).

    Stripping Membranes:

    Article Title: Proteomic analysis of flavokawain C-induced cell death in HCT 116 colon carcinoma cell line.
    Article Snippet: Accepted Manuscript Proteomic analysis of flavokawain C-induced cell death in HCT 116 colon carcinoma cell line Chung-Weng Phang, Nur Ashikhin Gandah, Sri Nurestri Abd Malek, Saiful Anuar Karsani PII: S0014-2999(19)30266-3 DOI: https://doi.org/10.1016/j.ejphar.2019.04.032 Reference: EJP 72351 To appear in: European Journal of Pharmacology Received Date: 26 December 2018 Revised Date: 12 April 2019 Accepted Date: 15 April 2019 Please cite this article as: Phang, C.-W., Gandah, N.A., Abd Malek, S.N., Karsani, S.A., Proteomic analysis of flavokawain C-induced cell death in HCT 116 colon carcinoma cell line, European Journal of Pharmacology (2019), doi: https://doi.org/10.1016/j.ejphar.2019.04.032.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Proteomic and Systematic Functional Profiling Unveils Citral Targeting Antibiotic Resistance, Antioxidant Defense, and Biofilm-Associated Two-Component Systems of Acinetobacter baumannii To Encumber Biofilm and Virulence Traits
    Article Snippet: Prior to isoelectric focusing (IEF), six immobilized pH gradient (IPG) strips (Immobiline DryStrip pH 4 to 7, 18 cm) were rehydrated for 12 h at 20°C by adding 350 μl (for each strip) of rehydration buffer (7 M urea, 2 M thiourea, 2% CHAPS, 0.5% IPG buffer [pH 4 to 7], 0.002% bromophenol blue, and 12.5 mg/ml Destreak reagent [GE Healthcare]) containing 500 μg of protein extracted from the control/citral-treated sample per strip (biological triplicates). .. After rehydration, IPG strips were placed on mineral oil in an IEF system (Ettan IPGphor 3; GE Healthcare), and IEF was carried out by applying a 75-μA current per IPG strip under the following conditions: 100 V (2-h step), 100 to 500 V (3-h gradient), 500 V (2-h step), 500 to 5,000 V (3-h gradient), 5,000 V (2-h step), 5,000 to 8,000 V (3-h gradient), 8,000 V (2-h step), 8,000 to 10,000 V (3-h gradient), and 10,000 V (2-h step) at 20°C for 22 h. Prior to two-dimensional gel electrophoresis (2DGE), the IPG strips were equilibrated with two different equilibration buffers, equilibration buffer I (6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 1% DTT in 50 mM Tris-HCl buffer [pH 8.8]) for 15 min and equilibration buffer II (6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 2.5% iodoacetamide [IAA] in 50 mM Tris-HCl buffer [pH 8.8]) for 15 min. Next, equilibrated IPG strips were placed on top of 12% gels and sealed with a 0.3% agarose solution. .. The Ettan DALTsix electrophoresis system (GE Healthcare) was used for electrophoresis under the following program conditions: 100 V (200 mA) for 1 h and 150 V (300 mA) for 8 h. After electrophoresis, the gels were fixed, stained by CBB according to the above-described method, and imaged at a 300-dpi (dots per inch) resolution using an Image Scanner III system (GE Healthcare) equipped with LabScan 6.0 software ( ).

    Electrophoresis:

    Article Title: Apparent degradation forms of rhG-CSF under forced conditions: Insights for better quality-control of bioproducts.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Generated:

    Article Title: Apparent degradation forms of rhG-CSF under forced conditions: Insights for better quality-control of bioproducts.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    other:

    Article Title: Comparative Pistacia vera leaf proteomics in response to herbivory of the common pistachio psylla (Agonoscena pistaciae)
    Article Snippet: Plants have evolved diverse mechanisms to cope with damages caused by insect herbivores.. Proteomics can greatly contribute to the understanding of plant changes in response to herbivory.. Here, two-dimensional polyacrylamide gel electrophoresis was used in a comparative proteomics approach to survey responses of pistachio seedlings to herbivory of the nymphal stages of the common pistachio psylla.

    Article Title: Melatonin ameliorates anxiety and depression-like behaviors and modulates proteomic changes in triple transgenic mice of Alzheimer's disease.
    Article Snippet: Alzheimer’s disease (AD) is a devastating neurodegenerative disease accompanied by neuropsychiatric symptoms, such as anxiety and depression.. The levels of melatonin decrease in brains of AD patients.. The potential effect of melatonin on anxiety and depression behaviors in AD and the underlying mechanisms remain unclear.

    Two-Dimensional Gel Electrophoresis:

    Article Title: Proteomic and Systematic Functional Profiling Unveils Citral Targeting Antibiotic Resistance, Antioxidant Defense, and Biofilm-Associated Two-Component Systems of Acinetobacter baumannii To Encumber Biofilm and Virulence Traits
    Article Snippet: Prior to isoelectric focusing (IEF), six immobilized pH gradient (IPG) strips (Immobiline DryStrip pH 4 to 7, 18 cm) were rehydrated for 12 h at 20°C by adding 350 μl (for each strip) of rehydration buffer (7 M urea, 2 M thiourea, 2% CHAPS, 0.5% IPG buffer [pH 4 to 7], 0.002% bromophenol blue, and 12.5 mg/ml Destreak reagent [GE Healthcare]) containing 500 μg of protein extracted from the control/citral-treated sample per strip (biological triplicates). .. After rehydration, IPG strips were placed on mineral oil in an IEF system (Ettan IPGphor 3; GE Healthcare), and IEF was carried out by applying a 75-μA current per IPG strip under the following conditions: 100 V (2-h step), 100 to 500 V (3-h gradient), 500 V (2-h step), 500 to 5,000 V (3-h gradient), 5,000 V (2-h step), 5,000 to 8,000 V (3-h gradient), 8,000 V (2-h step), 8,000 to 10,000 V (3-h gradient), and 10,000 V (2-h step) at 20°C for 22 h. Prior to two-dimensional gel electrophoresis (2DGE), the IPG strips were equilibrated with two different equilibration buffers, equilibration buffer I (6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 1% DTT in 50 mM Tris-HCl buffer [pH 8.8]) for 15 min and equilibration buffer II (6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 2.5% iodoacetamide [IAA] in 50 mM Tris-HCl buffer [pH 8.8]) for 15 min. Next, equilibrated IPG strips were placed on top of 12% gels and sealed with a 0.3% agarose solution. .. The Ettan DALTsix electrophoresis system (GE Healthcare) was used for electrophoresis under the following program conditions: 100 V (200 mA) for 1 h and 150 V (300 mA) for 8 h. After electrophoresis, the gels were fixed, stained by CBB according to the above-described method, and imaged at a 300-dpi (dots per inch) resolution using an Image Scanner III system (GE Healthcare) equipped with LabScan 6.0 software ( ).



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